Biocoupler™ Bundle

• Complete Starter Kit - The bundle includes six Biocoupler™ Glass Sets, 30 mL of Plant Preservative Mixture (PPM™), and 10 liters of Murashige & Skoog (MS) Media, providing all necessary materials to initiate tissue culture experiments immediately.
• Cost-Effective and Space-Efficient: Designed to be more affordable and occupy less space compared to traditional temporary immersion bioreactor systems, making it suitable for both hobbyists and large-scale plant businesses.
• Enhanced Contamination Prevention - The included PPM™ helps inhibit contamination from various sources, ensuring healthier cultures and reducing the risk of losses due to microbial interference.
• User-Friendly Design - The Biocoupler™ is easy to assemble and operate, requiring minimal technical expertise, which allows users to focus more on their research and propagation efforts.
• Durable design the Biocoupler™ is crafted from a high-strength material designed to withstand repeated high-temperature autoclave cycles. This construction ensures the device is virtually unbreakable under normal use, even if accidentally dropped onto a concrete floor.
• The Murashige & Skoog (MS) medium in the Biocoupler™ Bundle supports robust plant growth and shoot proliferation with its essential vitamins and nutrients. It is versatile for a wide variety of plant species, easy to prepare, and provides consistent, reliable results. This standardized nutrient base enhances cell division and tissue differentiation, aiding in successful in vitro plant propagation.

Biocoupler

• Biocoupler™ is used for temporary immersion and has been successfully tested for a wide range of plants, including orchids, hemp, aquatics, succulents, ferns, and carnivorous plants.
• It’s the simplest TIB system with a simple and elegant design, thus easy to operate.
• You don’t need to learn or have any prior experience before using Biocupler™ in your tissue culture system.
• It doesn’t require electricity to run.
• It’s more affordable and saves more space in your tissue culture lab. Thus, best for hobbyists, beginner culturists, or small-scale businesses. It’s also a great option for bigger labs based on their requirements.
• It works efficiently to multiply plants in hundreds or thousands under in vitro conditions.
• It’s a simple design and you only need to change its filter, which can commonly be found in any store, after some time—unlike traditional TIBs whose parts are often difficult to find.
• You don’t need to stress about the repair and maintenance of the Biocoupler™. Thus, full focus and investment go into your research or
  tissue culture operations.
• It does not need gelling agents, but does require MS media! Additionally, the use of PPM will help prevent contamination, benefiting plant growth. 

PPM™

• Controller of microbial airborne contamination.
• Manager of microbial waterborne contamination.
• Inhibitor of endogenous contamination.
• Enhancer of the success of culture experiments.
• Maintainer of the viability of in vitro seed germination.
• Promoter of callus proliferation.
• Supporter of callus regeneration.
• Ensurer of the stability of tissue culture media.

MS Medium

• In vitro plant growth and development
• Callus formation induction

Biocoupler™

1. Autoclave Biocouplers™ before use to avoid contamination. Utilizing PPM™ will help prevent contamination, benefiting plant growth. 
2. Select plant to multiply.
3. Breakdown explants and prepare multiplication media.
4. Add explants to sterilized Biocoupler™
5. Rotate on a scheduled based upon plant species (typically between once daily to once weekly).
6. Place Biocoupler™ in your growth area and monitor growth of explants.
7. Transfer plants to the appropriate state (multiplication, rooting, or acclimation) based on lab's need.

The BioTilt™ (patent pending) automates the rotation of your Biocouplers™ at precisely timed intervals, optimizing the growth conditions for plant multiplication without manual intervention.

Plant Preservative Mixture (PPM™)

1. General Dosage levels - With the exception of endogenous contamination, the recommended dose range is 0.05%-0.2%. (For callus proliferation, organogenesis and embryogenesis, the recommended range is 0.05-0.075%.) To eliminate higher endogenous contamination densities, higher doses of PPM™ are needed.
2. (a) For explants: gently and routinely shake / stir 1 cm. long explants (or shorter) for 4-12 hours in 4-5% v/v PPM™ solution supplemented as above with 3x MS strength basal salts without Tween 20.Without rinsing, insert into a medium supplemented with 0.05 - 0.1% PPM™ for herbaceous plants and 0.2% PPM™ for woody plants.  (b) F or ornamental plants only  tubers, bulbs and scales: shake / stir the entire tuber/ bulb / scale in bleach. Rinse with water (can be done under non-sterile conditions). Slice the tuber / bulb /scale to thin slices. Shake / stir for 12-24 hours in 4- 5% PPM™ solution supplemented with full strength basal salts without pH ing & Tween 20. Without rinsing, insert into a medium supplemented with 0.1 -0.2% PPM™.

Murashige & Skoog (MS) Medium

Follow the given steps to prepare 1 liter of tissue culture media using MS media:

1. Take a beaker and add 800 ml of distilled water to it.
2. To the water add PCT’s MS Media 4.54 grams, 30 grams of sugar, and PPM™ (generally 1-2 ml/L)
3. Make up the volume of the media to 1000ml. 
4. Adjust the pH of the media between 5.4-5.8.
5. Add gelling agent (3-4 g/L gellan gum or 6-8 g/L agar) to the media and keep stirring.
6. Autoclave the medium for 20 minutes at 15 psi and 121℃.
7. Your media is ready!

Note: MS Media should be prepared according to the formula mentioned on the label. However, it is recommended to use an entire container at once.