• White opaque bottle with yellow label containing DKW medium, also known as Driver and Kuniyuki Walnut medium, developed for walnut micropropagation with unique nitrogen and sulfate content for influencing plant growth.
  • Back of the Orchid Maintenance Media bottle. Upper left corner shows logo for Plant Cell Technology. Rest of the label contains information for contents and contact information for company. Warning labels for Fire Risk and Oxidizer are present. For laboratory use only.
  • xplore a large container of DKW Basal Medium with vitamins. The opaque white container features a vibrant yellow label red cap.
1 3

DKW Medium w/ Vitamins

DKW Medium w/ Vitamins

$19.99 USD
$19.99 USD $19.99 USD
Sale Sold out
Shipping calculated at checkout.
Size

DKW medium is also known as Driver and Kuniyuki Walnut medium. The media was originally developed for walnut micropropagation using nodal explant. Notably, DKW medium contains lower nitrogen and higher sulfate content, potentially influencing plant growth and development.

View full details

Benefits

  • Easy to use
  • Supply nutrients required by in vitro plants for their proper growth and development
  • Lower nitrogen content and higher sulfate content
  • Multiple shoot induction
  • Callus-mediated shoot regeneration

Applications

  • Multiple shoot induction
  • Callus-mediated shoot regeneration
  • Improved success rates for specific plant species (e.g., turmeric and walnut)
  • Used to tissue culture woody plants

How To Use

Follow the steps below to prepare 1 liter of tissue culture media using DKW media:

  1. Take a beaker and add 800 ml of distilled water to it.
  2. To the water add 5.36 grams of PCT’s DKW Media, 30 grams of sugar, and PPM™ (generally 1-2 ml/L).
  3. Make up the volume of the media to 1000ml.
  4. Adjust the pH of the media between 5.4-5.8.
  5. Add gelling agent (agar 6-8 g/L or gellan gum 3-4 g/L) to the media and keep stirring.=
  6. Autoclave the medium for 20 minutes at 15 psi and 121℃.
  7. Your media is ready!

  • Product Details

    1. Product Name DKW Medium Basal Medium with Vitamins
    2. Synonyms Driver and Kuniyuki Walnut medium
    3. Appearance White to light tan with a homogenous mixture of free-flowing powder
    4. Formula weight 5.36 g/L
    5. Solubility Water (Appear as colorless to slightly yellow solution, clear, complete)
    6. Suitability Pass for Tissue Culture
    7. pH (at 25°C) 4.8 ± 0.2
    8. Storage and Shelf Life Dehydrated plant tissue culture media is hygroscopic and should be protected from sunlight and moisture. Store the prepared medium at 2-8°C away from direct light. Medium should be used before the expiry date. The entire volume of each bottle should be used immediately after opening, or else the unused portion should be stored in desiccators and refrigerated at 2-8°C.