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Gellan Gum

Gellan Gum

$36.99 USD
$36.99 USD
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Gellan gum, an anionic polysaccharide, is employed as a replacement for agar in various clinical bacteriological and plant tissue culture media. Its structural composition comprises a repeating tetrasaccharide unit, including two D-glucose residues, one L-rhamnose residue, and one D-glucuronic acid residue.

It is an excellent alternative for nutrient media in plant tissue culture, yielding a clearer and more transparent gel than agar. Additionally, it requires only half the concentration of agar typically used in tissue culture media.

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Benefits

  • Easy to use
  • Acts as a solid support for the growth and development of explants
  • Allows the absorption of nutrients by the plant cells
  • The transparency of the gel allows one to clearly see the development of roots or contamination from the start.
  • Cost-effective.
  • Solidifying agents play a crucial role in tissue culture media by providing the necessary support to hold cells, tissues, or explants in place and ensure they receive optimal nutrients for growth and development.

Applications

  • Gellan Gum is used in doses ranging between 0.2% (2g/L) to 0.4% (4g/L) (w/v) to solidify plant tissue culture media.
  • Gellan Gum is used as a microbial solid culturing medium.

How To Use

  1. Take a beaker and add 800 ml of distilled water to it.
  2. To the water add PCT’s MS Media (or alternative media) 4.54 grams, 30 grams of sugar, and PPM™ (generally 1-2 ml/L).
  3. Make up the volume of the media to 1000ml. 
  4. Adjust the pH of the media between 5.4-5.8.
  5. Add 3-4 g/L of PCT’s Gellan Gum to the media and keep stirring.
  6. Autoclave the medium for 20 minutes at 15 psi and 121℃.
  7. Your media is ready!

  • Product Details

    1. Product Name Gellan Gum
    2. Synonyms Phytagel, Gelzan
    3. CAS No. 71010-52-1
    4. Shape Cylindrical container
    5. Material Powder
    6. Color Off White
    7. Strength >900 GM/CM^2