• White opaque bottle with yellow label containing MS or Murashige and Skoog Media, a balanced nutrient mixture developed by Toshio Murashige and Folke Skoog for optimal plant tissue culture.
  • Back of the Orchid Maintenance Media bottle. Upper left corner shows logo for Plant Cell Technology. Rest of the label contains information for contents and contact information for company. Warning labels for Fire Risk and Oxidizer are present. For laboratory use only.
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Murashige & Skoog (MS) Basal Medium w/ Vitamins

Murashige & Skoog (MS) Basal Medium w/ Vitamins

$15.99 USD
$15.99 USD
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Murashige and Skoog Media, MS Media is a nutrient media used for the in vitro culture of plant cells, tissues, and organs. It was first developed in 1962 by Toshio Murashige and Folke Skoog and has since become one of the most widely used media for plant tissue culture.

It contains a balanced mixture of macronutrients, micronutrients, vitamins, and plant growth regulators to provide optimal conditions for the proper growth and development of plants.

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Benefits

  • Easy to use
  • Supply nutrients required by in vitro plants for their proper growth and development
  • Provides a well-balanced composition of nutrients, including essential minerals and vitamins, supporting the growth of various plant tissues.
  • Effective in inducing callus formation
  • A dynamic supplier of numerous amino acids, organic acids, plant growth regulators (such as cytokinins and auxins), vitamins, sugars, minerals, nucleic acids, and unidentified growth-promoting substances.

Applications

  • In vitro plant growth and development
  • Callus formation induction

How To Use

  1. Take a beaker and add 800 ml of distilled water to it.
  2. To the water add PCT’s MS Media 4.54 grams, 30 grams of sugar, and PPM™ (generally 1-2 ml/L)
  3. Make up the volume of the media to 1000ml. 
  4. Adjust the pH of the media between 5.4-5.8.
  5. Add gelling agent (3-4 g/L gellan gum or 6-8 g/L agar) to the media and keep stirring.
  6. Autoclave the medium for 20 minutes at 15 psi and 121℃.
  7. Your media is ready!

Note: Media should be prepared according to the formula mentioned on the label. However, it is recommended to use an entire container at once. 

Heat-liable substrates should be added, after autoclaving.

  • Product Details

    1. Product Name Murashige & Skoog (MS) Basal Medium w/Vitamins.
    2. Synonyms MS Basal Medium
    3. Shelf Life See expiry date on the bottle
    4. Appearance Homogenous mixture of free-flowing powder
    5. Color White to light tan
    6. Shape Cylindrical container
    7. Material Liquid
    8. Solubility Partially soluble in cold water and completely soluble in hot water