Starter Kit

• Comprehensive Kit for Beginners - Provides all essential components to help new users easily start plant tissue culture.
• MS Media with Vitamins - Supports robust plant growth and shoot proliferation, with the ability to prepare 5 liters of tissue culture media.
• High-Quality Agar - Supreme tissue-culture-grade agar ensures optimal clarity and support for plant tissue growth.
• Contamination Prevention - Includes 10 ml of Plant Preservative Mixture (PPM™) to effectively prevent or reduce bacterial and fungal contamination.
• Optimal Growth Environment - Comes with 2 squared culture vessels that are transparent, autoclavable, and designed for efficient plant growth.
• Easy Ingredient Measurement - Includes a disposable syringe for accurate and simple media preparation.

• The PCT Starter Kit facilitates propagation of rare species, genetic modifications, and advanced plant biology techniques for enthusiasts and researchers alike.
• Supreme TC Grade Agar - Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.

Murshige & Skoog Medium

• Dissolve 4.54gms of dehydrated medium in 600ml of distilled or deionized water at room temperature (15-30*C).
• Rinse media vial with small quantity of distilled water to remove traces of power.
• Add the desired heat stable supplements prior to autoclaving.
• Continue stirring until the powder has dissolved. Some times media does not dissolve completely unless the pH is reduced. For these, lower the pH to about 3.0 to facilitate dissolution of media. The pH of medium is adjusted by using 1N HCL/ 1N NaOH/ 1NKOH.
• Make up the final volume to 1000ml with distilled water.
• Mix Gently, heat and rotate between intervals until the solution becomes clear. Do not boil, reheat and allow to cool below 50*C during dispensing.
• Dispense the medium into suitable containers, plug or cap, then autoclave at 15pso (121*C) for 15 minutes, using a slow exhaust cycle. Higher temperatures and/or longer times are not recommended.
• Cool the autoclaved culture vessels containing medium to 45-50*C and aseptically add desired sterile heat-labile substrate.

Note: Media should be prepared according to formula mentioned on the label, however, it is recommended to use an entire container at once. Heat-liable substrates should be added, after autoclaving.

Plant Preservative Mixture

1. General Dosage levels - With the exception of endogenous contamination, the recommended dose range is 0.05%-0.2%. (For callus proliferation, organogenesis and embryogenesis, the recommended range is 0.05-0.075%.) To eliminate higher endogenous contamination densities, higher doses of PPM™ are needed.
2. For explants - gently and routinely shake / stir 1 cm. long explants (or shorter) for 4-12 hours in 4-5% v/v PPM™ solution supplemented as above with 3x MS
   strength basal salts without Tween 20.Without rinsing, insert into a medium supplemented with 0.05 - 0.1% PPM™ for herbaceous plants and 0.2% PPM™ for woody plants. For ornamental plants, ornamental plants, tubers, bulbs and scales: shake / stir the entire tuber/ bulb / scale in bleach. Rinse with water (can be done under non-sterile conditions). Slice the tuber / bulb /scale to thin slices. Shake / stir for 12-24 hours in 4- 5% PPM™ solution supplemented with full strength basal salts without pH ing & Tween 20. Without rinsing, insert into a medium supplemented with 0.1 -0.2% PPM™.

Supreme TC Agar

Follow the given steps to prepare 1 liter of tissue culture media using PCT’s Supreme TC Agar:

1. Take a beaker and add 800 ml of distilled water to it.
2. To the water add 4.54 grams of PCT’s MS Media (or alternative medium based on your plant), 30 grams of sugar, and PPM™ (generally 1-2 ml/L).
3. Make up the volume of the media to 1000ml. 
4. Adjust the pH of the media between 5.4-5.8.
5. Add 6-8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
6. Autoclave the medium for 20 minutes at 15 psi and 121℃.
7. Your media is ready!