• White opaque bottle with green label showcasing Supreme Grade Agar, a solidifying agent derived from seaweed, crucial for optimal growth and differentiation of plant cells in tissue culture.
  • Supreme TC Grade Agar displaying a plant specimen embedded in agar.
  • Supreme TC Grade Agar displaying a plant specimen embedded in agar.
  • Supreme TC Grade Agar displaying empty vessel of agar.
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Supreme TC Grade Agar

Supreme TC Grade Agar

$19.99 USD
$19.99 USD
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Solidifying agents play a vital role in tissue culture media, providing essential support for the optimal growth and development of plants when incorporated into the medium. Additionally, the inclusion of solidifying agents helps ensure the even distribution of nutrients, hormones, and other crucial elements throughout the culture medium.

The two most common solidifying agents used in the process are agar and gellan gum. Agar, derived from seaweed, is a polysaccharide. It imparts a gel-like texture to the medium, facilitating the adherence, growth, and differentiation of plant cells.

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Benefits

  • Easy to use.
  • Acts as a solid support for the growth and development of explants.
  • Allows the absorption of nutrients by the plant cells
  • Cost-effective.

Applications

  • Agar is used as microbial solid culturing media.
  • The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media.
  • Agar is an essential ingredient used for in vitro root development.

How To Use

  1. Take a beaker and add 800 ml of distilled water to it.
  2. To the water add 4.54 grams of PCT’s MS Media (or alternative medium based on your plant), 30 grams of sugar, and PPM™ (generally 1-2 ml/L).
  3. Make up the volume of the media to 1000ml. 
  4. Adjust the pH of the media between 5.4-5.8.
  5. Add 6-8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
  6. Autoclave the medium for 20 minutes at 15 psi and 121℃.
  7. Your media is ready!

  • Product Details

    1. Product Name Supreme TC Grade Agar
    2. Synonyms Gelose, Agar Agar Agar
    3. CAS No. 9002-18-0
    4. Capacity Available in different weights of 100gm, 500gm, 1Kg, and 5Kg
    5. Shape Cylindrical container
    6. Material Powder
    7. Color Light beige
    8. Solubility Partially soluble in cold water and completely soluble in hot water
    9. Gelling Strength 980 g/cm2